ABSTRACT
Renal disorders have become very common nowadays, which may also lead to kidney failure. The disorder may be caused by the commonly used chemicals such as acetaminophen, CCl4, streptromycin, H2O2 etc. The objective of this work is to determine the nephroprotective potential of ethanolic extract against H2O2 induced toxicity in VERO cell line. Ethanolic extract is known for its antioxidant, anti-inflammatory and anti-microbial effects, which make it a most sought for herbal medicine. Its characteristic features have identified this compound as a potential hepatoprotective and nephroprotective agent. VERO cells are cells taken from the kidney of an African green monkey, which are used in our study. The matured leaves of Melia Azadirachta were used to prepare ethanolic extract and the same was used to test for its inhibitory effect in 96 micro plate formats against in VERO cell lines. To study the cytotoxic properties of ethanolic extract against VERO cell line, we have tested the MTT assay with different concentrations in the range of 1000 to 62.5 μg/ml. From the performed assay, the effect of ethanolic extract drug reveals an enhanced activity on in VERO cell lines and that infers Melia Azadirachta, can be used as nephroprotective agent.
ABSTRACT
To quantify the colchicine (alkaloid) present in Gloriosa superba by High Performance Liquid Chromatography (HPLC) method. Gloriosa superba species are known for their content of colchicine, a valuable alkaloid in the seeds and tubers (corms). HPLC analysis was carried out to confirm the presence of colchicine in Gloriosa superba. Treatment of gibberellic acid and Psedomonas aerugenosa improved the content of colchicine in seeds and tubers. Seeds of Gloriosa superba were rich in colchicines content than the tubers sample.